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rabbit anti-orm1  (Proteintech)


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    Proteintech rabbit anti-orm1
    Rabbit Anti Orm1, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+orm1+antibody/orm1+rabbit+polyclonal+antibody/pm40464660-185-25-20
    Average 90 stars, based on 1 article reviews
    rabbit anti-orm1 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Contribution of the α 1 -Acid Glycoprotein in Drug Pharmacokinetics: The Usefulness of α 1 -Acid Glycoprotein-Knockout Mice.
    Article Snippet: α1-Acid glycoprotein (AGP) is a primary binding protein for many basic drugs in plasma.. The number of drugs that bind to AGP, such as molecular target anticancer drugs, has been continuously increasing.. Since the plasma level of AGP fluctuates under various pathological conditions such as inflammation, it is important to evaluate the contribution of AGP to drug pharmacokinetics.

    Saline:

    Article Title: Contribution of the α 1 -Acid Glycoprotein in Drug Pharmacokinetics: The Usefulness of α 1 -Acid Glycoprotein-Knockout Mice.
    Article Snippet: α1-Acid glycoprotein (AGP) is a primary binding protein for many basic drugs in plasma.. The number of drugs that bind to AGP, such as molecular target anticancer drugs, has been continuously increasing.. Since the plasma level of AGP fluctuates under various pathological conditions such as inflammation, it is important to evaluate the contribution of AGP to drug pharmacokinetics.

    Incubation:

    Article Title: An acute phase protein α 1 -acid glycoprotein mitigates AKI and its progression to CKD through its anti-inflammatory action
    Article Snippet: .. After washing 3 times with 0.05% PBS-T, the primary antibody reaction with rabbit anti-ORM1 antibody (1:5000, Proteintech, IL) was incubated overnight at 4 °C. ..



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    The primer sequences
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    Image Search Results


    The primer sequences

    Journal: Bioengineered

    Article Title: Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9

    doi: 10.1080/21655979.2021.1987067

    Figure Lengend Snippet: The primer sequences

    Article Snippet: A polymer reinforcer was added dropwise and incubated at room temperature for 20 min. Rabbit anti-ORM1 polyclonal antibody (diluted 1:200, Proteintech) was added and incubated at room temperature for 30 min. After incubating with DAB solution, the slides were observed by microscopy.

    Techniques:

    The expression of ORM1 is upregulated in breast cancer a IHC staining of the tissues derived from the patients. b The expression level of ORM1 mRNA in the breast cancer patients’ tissues and the adjacent normal tissues. Data are presented as mean ± SEM from three independent experiments, n = 10, respectively. **** P < 0.0001. c The expression of ORM1 was reanalyzed from the previously published dataset GSE58812. d The expression level of ORM1 mRNA in the normal breast cell line HBL-100 and the breast cancer cell lines. Data are presented as mean ± SEM from three independent experiments. ** P < 0.01; **** P < 0.0001, compared with the HBL-100 cell line

    Journal: Bioengineered

    Article Title: Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9

    doi: 10.1080/21655979.2021.1987067

    Figure Lengend Snippet: The expression of ORM1 is upregulated in breast cancer a IHC staining of the tissues derived from the patients. b The expression level of ORM1 mRNA in the breast cancer patients’ tissues and the adjacent normal tissues. Data are presented as mean ± SEM from three independent experiments, n = 10, respectively. **** P < 0.0001. c The expression of ORM1 was reanalyzed from the previously published dataset GSE58812. d The expression level of ORM1 mRNA in the normal breast cell line HBL-100 and the breast cancer cell lines. Data are presented as mean ± SEM from three independent experiments. ** P < 0.01; **** P < 0.0001, compared with the HBL-100 cell line

    Article Snippet: A polymer reinforcer was added dropwise and incubated at room temperature for 20 min. Rabbit anti-ORM1 polyclonal antibody (diluted 1:200, Proteintech) was added and incubated at room temperature for 30 min. After incubating with DAB solution, the slides were observed by microscopy.

    Techniques: Expressing, Immunohistochemistry, Derivative Assay

    ORM1 promote the proliferation, migration and epirubicin resistance of breast cancer cells a Colony formation assay was performed to detect the proliferation of the cell lines, scale bar: 5 μ m. b Transwell assay was performed to detect the migration of the cell lines, scale bar: 50 μ m. c CCK-8 assay was performed to analyze the epirubicin IC50 values of the cell lines. d Data are presented as mean ± SEM from three independent experiments. *** P < 0.001; **** P < 0.0001, compared with the HBL-100 cell line

    Journal: Bioengineered

    Article Title: Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9

    doi: 10.1080/21655979.2021.1987067

    Figure Lengend Snippet: ORM1 promote the proliferation, migration and epirubicin resistance of breast cancer cells a Colony formation assay was performed to detect the proliferation of the cell lines, scale bar: 5 μ m. b Transwell assay was performed to detect the migration of the cell lines, scale bar: 50 μ m. c CCK-8 assay was performed to analyze the epirubicin IC50 values of the cell lines. d Data are presented as mean ± SEM from three independent experiments. *** P < 0.001; **** P < 0.0001, compared with the HBL-100 cell line

    Article Snippet: A polymer reinforcer was added dropwise and incubated at room temperature for 20 min. Rabbit anti-ORM1 polyclonal antibody (diluted 1:200, Proteintech) was added and incubated at room temperature for 30 min. After incubating with DAB solution, the slides were observed by microscopy.

    Techniques: Migration, Colony Assay, Transwell Assay, CCK-8 Assay

    Downregulation of ORM1 suppressed the malignant phenotype of breast cancer cells a qRT-PCR assay was performed to detect the transfection efficiency on si-ORM1; b Colony formation was performed to detect the proliferation after downregulating ORM1, scale bar: 5 μ m; c Wound healing assay was performed to detect the migration at different time after downregulating ORM1; d Flow cytometry was performed to detect the apoptosis of cells treating with epirubicin after downregulating ORM1

    Journal: Bioengineered

    Article Title: Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9

    doi: 10.1080/21655979.2021.1987067

    Figure Lengend Snippet: Downregulation of ORM1 suppressed the malignant phenotype of breast cancer cells a qRT-PCR assay was performed to detect the transfection efficiency on si-ORM1; b Colony formation was performed to detect the proliferation after downregulating ORM1, scale bar: 5 μ m; c Wound healing assay was performed to detect the migration at different time after downregulating ORM1; d Flow cytometry was performed to detect the apoptosis of cells treating with epirubicin after downregulating ORM1

    Article Snippet: A polymer reinforcer was added dropwise and incubated at room temperature for 20 min. Rabbit anti-ORM1 polyclonal antibody (diluted 1:200, Proteintech) was added and incubated at room temperature for 30 min. After incubating with DAB solution, the slides were observed by microscopy.

    Techniques: Quantitative RT-PCR, Transfection, Wound Healing Assay, Migration, Flow Cytometry

    ORM1 suppressed the migration of breast cancer cells via targeting MMP-2 and MMP-9 a -PCR assay and western blot were performed to detect the mRNA expression of MMP-2 and MMP-9 after downregulating ORM1; b Western blot was performed to detect the expression of MMP-2 and MMP-9 after upregulating MMP-2 or MMP-9; c Transwell assay was performed to detect the migration after upregulating MMP-2 and MMP-9, respectively

    Journal: Bioengineered

    Article Title: Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9

    doi: 10.1080/21655979.2021.1987067

    Figure Lengend Snippet: ORM1 suppressed the migration of breast cancer cells via targeting MMP-2 and MMP-9 a -PCR assay and western blot were performed to detect the mRNA expression of MMP-2 and MMP-9 after downregulating ORM1; b Western blot was performed to detect the expression of MMP-2 and MMP-9 after upregulating MMP-2 or MMP-9; c Transwell assay was performed to detect the migration after upregulating MMP-2 and MMP-9, respectively

    Article Snippet: A polymer reinforcer was added dropwise and incubated at room temperature for 20 min. Rabbit anti-ORM1 polyclonal antibody (diluted 1:200, Proteintech) was added and incubated at room temperature for 30 min. After incubating with DAB solution, the slides were observed by microscopy.

    Techniques: Migration, Western Blot, Expressing, Transwell Assay

    ORM1 could upregulate the MMP-2 and MMP-9 then activate the AKT/Erk signaling pathways A-B. Western blot assay was performed to detect the level of ORM1, p-AKT, AKT, p-Erk and Erk

    Journal: Bioengineered

    Article Title: Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9

    doi: 10.1080/21655979.2021.1987067

    Figure Lengend Snippet: ORM1 could upregulate the MMP-2 and MMP-9 then activate the AKT/Erk signaling pathways A-B. Western blot assay was performed to detect the level of ORM1, p-AKT, AKT, p-Erk and Erk

    Article Snippet: A polymer reinforcer was added dropwise and incubated at room temperature for 20 min. Rabbit anti-ORM1 polyclonal antibody (diluted 1:200, Proteintech) was added and incubated at room temperature for 30 min. After incubating with DAB solution, the slides were observed by microscopy.

    Techniques: Protein-Protein interactions, Western Blot